| CTGF

Connective tissue growth factor (CTGF), also known as CCN2 , is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. Members of the CCN protein family, including CTGF, are structurally characterized by having four conserved, cysteine-rich domains. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.1	1.86	1.36	0.94	0.57	0.36	0.25	0.09
Corrected OD	2.01	1.77	1.27	0.85	0.48	0.27	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat CTGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-109	105

 

| Linearity

Samples were spiked with high concentrations of Rat CTGF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-97	88-96	90-110
	Average (%)	92	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108